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Breaking Taboo, Swedish Scientist Seeks To Edit DNA Of Healthy Human Embryos

Sep 22, 2016
Originally published on December 21, 2016 4:02 pm

A scientist in Sweden has started trying to edit the DNA in healthy human embryos, NPR has learned.

The step by the developmental biologist Fredrik Lanner makes him the first researcher known to attempt to modify the genes of healthy human embryos. That has long been considered taboo because of safety and ethical concerns.

Lanner is attempting to edit genes in human embryos to learn more about how the genes regulate early embryonic development. He hopes the work could lead to new ways to treat infertility and prevent miscarriages. He also hopes to help scientists learn more about embryonic stem cells so they can someday use them to treat many diseases.

The fear is that Lanner's work could open the door to others attempting to use genetically modified embryos to make babies.

Making changes to the DNA in human embryos could accidentally introduce an error into the human gene pool, inadvertently creating a new disease that would be passed down for generations, critics say.

Some also worry the experiments could open the door to so-called designer babies that would let parents pick and choose the traits of their children.

Lanner, however, says he is initially planning only to study the modified embryos for the first seven days of their growth and would never let them develop past 14 days. The potential benefits could be enormous, he argues.

"Having children is one of the major drives for a lot of people," Lanner says. "For people who do struggle with this, it can tend to become an extremely important part of your life."

Lanner also hopes to learn things that could help scientists who are trying to turn stem cells from human embryos into new treatments for diseases.

"If we can understand how these early cells are regulated in the actual embryo, this knowledge will help us in the future to treat patients with diabetes, or Parkinson, or different types of blindness and other diseases," he says. "That's another exciting area of research."

NPR recently got exclusive access to Lanner's labs at the Karolinska Institute in Stockholm to watch some of his early efforts.

During the visit, Lanner and a graduate student carefully thawed five embryos donated by couples who had gone through in vitro fertilization at the Karolinska University Hospital to try to have children.

One of the embryos didn't survive the freezing and thawing process. The researchers gingerly placed each of the remaining 2-day-old embryos into a dish on a special microscope.

"You need to be stable on your fingers and hands while doing this," Lanner said, quipping, "You don't want to be dropping the embryos while taking them out."

With Lanner looking on, the student injected one of each embryo's four cells with a genetic engineering tool known as CRISPR-Cas9 while holding the embryo in place with a thin glass rod.

The gene-editing tool comprises two molecules that can zero in on individual genes and make very precise changes to the DNA. It lets scientists modify DNA much more easily and precisely than ever before. Lanner calls the technique a "game changer."

"It's not just quicker or cheaper," Lanner says. "This actually opens the door to start to look at this for the first time, because we could not do this at all previously in the human embryo. The technology was just not efficient enough to try to look at individual gene function as the embryo develops."

Lanner is planning to methodically knock out a series of genes that he has identified through previous work as being crucial to normal embryonic development. He hopes that will help him learn more about what the genes do and which ones cause infertility.

He declined to specify which genes he's targeting until the work is reviewed and published.

During the visit by NPR, one of the embryos got severely damaged when the injection needle got clogged. But the researchers successfully injected the remaining three embryos and placed them in an incubator to continue developing. One embryo divided again immediately after being injected, showing that it could still grow.

Two of the embryos survived in good enough shape to be analyzed later, Lanner explained in an email afterward.

Lanner has now done this on at least a dozen embryos, but is still studying his results and refining his techniques. He remains unsure how well the editing is working so far. However, he's confident he'll be able to modify individual genes in the embryos to determine their function.

"It will be very exciting. We're fortunate to be in this position," Lanner says. "This is a privilege to be in this position."

But just the act of attempting to edit the DNA in healthy human embryos is extremely controversial. Chinese scientists triggered an international uproar earlier last year when they tried to edit the DNA of human embryos even though they used only defective embryos that had no hope of developing.

Experiments like these intensified calls for a moratorium on such research, and the National Academies of Sciences, Engineering and Medicine launched the Human Gene-Editing Initiative to sort through the complex scientific and ethical issues they raise.

Organizers of an international summit convened in Washington, D.C., last year as part of that process concluded that it was far too early to try to create a baby from embryos that had their genes edited.

But the organizers said basic research like Lanner's could be acceptable. A final report from the gene-editing initiative is expected late this year or early next.

Still, not everyone agreed with the summit organizers' assessment. Some people have moral objections to doing any research on human embryos because they consider a human embryo to have the moral standing of a person.

And editing the DNA in embryos is controversial even among people who think human embryonic research is acceptable. That's the position of Marcy Darnovsky, who heads the Center for Genetics & Society, a watchdog group based in California that supports human embryonic research.

"The production of genetically modified human embryos is actually quite dangerous," Darnovsky says. "It's a step toward attempts to produce genetically modified human beings. This would be reason for grave concern."

One fear is that scientists could make some kind of mistake, accidentally creating new diseases that would be passed down for generations.

"When you're editing the genes of human embryos, that means you're changing the genes of every cell in the bodies of every offspring, every future generation of that human being," Darnovsky says. "So these are permanent and probably irreversible changes that we just don't know what they would mean."

But even if it's safe, Darnovsky and others still worry about what designer babies would do to society.

"If we're going to be producing genetically modified babies, we are all too likely to find ourselves in a world where those babies are perceived to be biologically superior. And then we're in a world of genetic haves and have-nots," Darnovsky says. "That could lead to all sorts of social disasters. It's not a world I want to live in."

Lanner says he has no interest in ever doing anything like that. In fact, at the moment it would be illegal in Sweden. And, Lanner says, much more research would be needed to make sure it would be safe before anyone tries to use a genetically modified embryo to make a baby to prevent diseases.

"It's not a technology that should be taken lightly," he says. "So I really, of course, stand against any sort of thoughts that one should use this to design designer babies or enhance for aesthetic purposes."

But Lanner argues that basic research is necessary and morally acceptable, and banning it would be counterproductive.

"I think it's wise to be allowed to do fundamental research so we can gain more information about this technology and potentially use it in the future," he says.

Lanner plans to continue attempting to edit the DNA in healthy human embryos until he develops efficient editing techniques that will allow him to study the genes involved in early embryonic development. Scientists in Britain are planning to start similar experiments later this year.

Research using human embryos is legal in the U.S., but not with the support of federal funds. U.S. labs that are known to be active in human embryo research have not announced any plans to proceed with gene-editing experiments.

Copyright 2016 NPR. To see more, visit http://www.npr.org/.

STEVE INSKEEP, HOST:

NPR's learned that a scientist in Sweden is trying something sure to be controversial. He is the first scientist in the world known to be trying to genetically modify healthy human embryos, which raises many questions about how far this science is going to go. NPR health correspondent Rob Stein is breaking this story.

Hi, Rob.

ROB STEIN, BYLINE: Hi, Steve. Nice to be here.

INSKEEP: I'm glad you are here. Who is this scientist and what exactly is he trying to do?

STEIN: So his name is Fredrik Lanner. And he's a developmental biologist at the Karolinska Institutet in Stockholm. And he's using a brand new kind of genetic engineering that allows scientists to make changes in the DNA in human embryos in ways that were never possible before.

INSKEEP: Why does he want to do that?

STEIN: So he wants to do it for basic medical research. He has two goals, really. One is to find new ways to treat infertility and prevent miscarriages by understanding the genes that control early human embryonic development. The second one is to learn more about human embryonic stem cells, which scientists want to use the treat all kinds of incurable diseases like Alzheimer's and Parkinson's and blindness and diabetes. By understanding the genetics of early embryos, he hopes to help them do that.

INSKEEP: OK. That all sounds great. Why are people so worried about something like this happening?

STEIN: Well, the fear is that somebody might take this further and try to create genetically modified human beings using genetically modified human embryos. And the concern there is that they could make some kind of mistake. I mean, this is new science. They don't really know how well it works. They could accidentally create a new disease that would then be passed down for generations and kind of mess up the human gene pool for generations.

INSKEEP: This isn't the same way that people do genetically modified plants. We're talking about genetically modified people.

STEIN: Well, that's the fear - is that they could use these techniques to create, someday, genetically modified people. You know, designer babies, where parents pick and choose the traits of their babies - make them taller or stronger or smarter, something like that - we're nowhere near being able to do that. But the concern is that this could open the door to, someday, somebody trying that.

INSKEEP: So NPR's Rob Stein is reporting, again, that a scientist is working with human embryos to modify them for the first time for medical research.

He let you into his lab, first reporter to be in there. What was it like?

STEIN: Yeah, so I traveled to Stockholm, and I went to his lab. And his labs are right upstairs from an IVF clinic. And that's what he's using are leftover embryos from couples who went through IVF to try to have babies. These are very early embryos - only 2 days old, only four cells. And it was fascinating to watch. Everything they were doing was projected onto a monitor and magnified. And you could see these tiny embryos in the dish under the microscope and this tiny little needle piercing the shell of the embryo so they can inject this genetic engineering tool I mentioned. Lanner refers to it as kind of a genetic molecular scissor that cuts the DNA in very precise places.

INSKEEP: Wow.

STEIN: Let's listen a little bit as Lanner looks over the shoulder of his assistant as he tries to edit one of these embryos.

FREDRIK LANNER: So now he just injected the second embryo. And you can see the little area there where the solution has been injected. And that's where we would have the scissors.

STEIN: That's how you edit the DNA in a human embryo - that's what you just did. That's how you go about doing it?

LANNER: Yeah, yeah.

INSKEEP: I'm just trying to think of tiny scissors small enough to fit inside one human cell. This is amazing.

STEIN: Yes.

INSKEEP: But what did he say when you raised the ethical concerns that we're talking about here?

STEIN: Lanner said, well, look it - listen, I have no intention of doing any those sci-fi kinds of things that people are worried about. All I want to do is basic research to help women have babies. Let's listen a little bit what Lanner said when I talked to him about these concerns.

LANNER: I think this is something with all new technology - that it has good use and it could have sort of use that is not morally acceptable - right? - and designing babies or making sure you have a blue-eyed baby or something similar. The only way we can sort of prevent that from happening is to have a - good laws regulating this.

STEIN: And in fact, Lanner told me that right now, at least, in Sweden, it would be illegal to try to make a human being from a genetically modified embryo. But that's not the case in all parts of the world. There are countries where it would be legal. And there are other scientists in other parts of the world who are starting to do experiments using genetic modification on human embryos.

INSKEEP: The same way that this man is?

STEIN: Yeah, they do similar kind of basic research right now.

INSKEEP: Rob, thanks very much for bringing us this story.

STEIN: Sure. Great to be here. Transcript provided by NPR, Copyright NPR.